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Biochemistry of homologous recombination
Characterization
of recombinase function in vivo
Characterization
of DNA intermediates in meiotic recombination
Genetic dissection
of BRCA1 function
Intergenic suppression
of BRCA1 function
Characterization
of drugs that alter the efficiency of homologous recombination
Our collaborator Dr.
Philip Connell conducted
a small molecule screen for compounds that alter the DNA binding
properties of recombinase Rad51. The screen identified compounds
that both stimulate and inhibit Rad51’s ability to bind
DNA. Our laboratory is working with Dr. Connell’s group
to characterize the mechanisms through which these molecules alter
Rad51. Thus far we have shown that one molecule identified on
the basis of enhanced DNA binding also elevates recombination
activity in a D-loop assay. Future efforts will be directed at
developing this and related compounds for use in chemotherapy
and gene therapy.
Effect of a small
molecule Rad51-DNA binding enhancer on the D-loop activity of
human Rad51 protein. (The method is described in Hong
et al. J Biol Chem. 2001 Nov 9;276(45):41906-12.)
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