| Biochemistry
of homologous recombination 
Characterization
of recombinase function in vivo
Characterization
of DNA intermediates in meiotic recombination
Genetic dissection
of BRCA1 function
Intergenic suppression
of BRCA1 function
Characterization
of drugs that alter the efficiency of homologous recombination
BRCA1 is a huge protein known
to associate with a wide variety of proteins. Some of these interacting
proteins are known to contribute to recombinational repair of
DSB while others are involved in disparate processes. To understand
the functional domains of BRCA1, we established a method that
allows us to “knock-in” mutant alleles in DT40 cells.
The resulting mutant lines are tested for recombination efficiency
in a variety of assays including immunoglobin gene conversion,
gene targeting, and sister chromatid exchange. The goal of this
work is to determine the domains of the protein necessary and
sufficient for each of its activities.
Diagram shows regions
of BRCA1 shown (by other labs) to bind the proteins indicated.
Aqua colored segments indicate conserved motifs that our lab identified
(
Orelli et al. Oncogene. 2001 Jul 19;20(32):4433-8.)
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